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- KD/KO Validated
OIP5 Polyclonal antibody
OIP5 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12142-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 10 publications below |
| IHC | See 5 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
Product Information
12142-1-AP targets OIP5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OIP5 fusion protein Ag2790 |
| Full Name | Opa interacting protein 5 |
| Calculated Molecular Weight | 229 aa, 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC015050 |
| Gene Symbol | OIP5 |
| Gene ID (NCBI) | 11339 |
| RRID | AB_2157076 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OIP5, also named as IS18B and CT86, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It is over expressed in the majority of primary lung and esophageal cancers. OIP5 is a promising target for developing new prognostic biomarkers and anti-cancer drugs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OIP5 antibody 12142-1-AP | Download protocol |
| IP protocol for OIP5 antibody 12142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Bull (Beijing) Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer | ||
Neuro Oncol Cancer-testis specific gene OIP5: a downstream gene of E2F1 that promotes tumorigenesis and metastasis in glioblastoma by stabilizing E2F1 signaling.
| ||
Oncotarget OIP5, a target of miR-15b-5p, regulates hepatocellular carcinoma growth and metastasis through the AKT/mTORC1 and β-catenin signaling pathways. | ||
J Biol Chem Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone HJURP.
| ||
Int J Oncol Characterization of Opa interacting protein 5 as a new biomarker and therapeutic target for oral cancer.
| ||
Cancer Sci Characterization of an Opa interacting protein 5 involved in lung and esophageal carcinogenesis. |
