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- KD/KO Validated
ONECUT2 Polyclonal antibody
ONECUT2 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, ChIP, Cell treatment, ELISA
Conjugate
Unconjugated
Cat no : 21916-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, mouse skin tissue, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
| ChIP | See 3 publications below |
Product Information
The immunogen of 21916-1-AP is ONECUT2 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ONECUT2 fusion protein Ag16162 |
| Full Name | one cut homeobox 2 |
| Calculated Molecular Weight | 504 aa, 54 kDa |
| Observed Molecular Weight | 54-60 kDa |
| GenBank Accession Number | BC108925 |
| Gene Symbol | ONECUT2 |
| Gene ID (NCBI) | 9480 |
| RRID | AB_2848180 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ONECUT2, also named as One cut domain family member 2 or Hepatocyte nuclear factor 6-beta, is a 504 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT2 belongs to the CUT homeobox family and activates the transcription of a number of liver genes such as HNF3B. ONECUT2 is required for liver differnetiation and metabolism. ONECUT2 and ONECUT3 play redundant roles with ONECUT1 in pancreas development.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ONECUT2 antibody 21916-1-AP | Download protocol |
| IP protocol for ONECUT2 antibody 21916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Temporal evolution reveals bifurcated lineages in aggressive neuroendocrine small cell prostate cancer trans-differentiation | ||
Nat Med ONECUT2 is a targetable master regulator of lethal prostate cancer that suppresses the androgen axis. | ||
Cancer Cell Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma. | ||
Sci Adv Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression. | ||
Proc Natl Acad Sci U S A Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.
| ||
Cell Death Dis ONECUT2 facilitates hepatocellular carcinoma metastasis by transcriptionally upregulating FGF2 and ACLY
|
