ORAI1 Monoclonal antibody

ORAI1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C12F9

Cat no : 66223-1-Ig

Synonyms

ORAI1



Tested Applications

Positive WB detected inHL-60 cells, 4T1 cells, A375 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, pig thymus tissue, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells
Positive IF detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66223-1-Ig targets ORAI1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, monkey, bovine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ORAI1 fusion protein Ag18050
Full Name ORAI calcium release-activated calcium modulator 1
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC015369
Gene Symbol ORAI1
Gene ID (NCBI) 84876
RRIDAB_2881614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Protocols

Product Specific Protocols
WB protocol for ORAI1 antibody 66223-1-IgDownload protocol
IF protocol for ORAI1 antibody 66223-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Death Dis

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats

Authors - Yajie Wang
humanWB,IF

Oncogenesis

Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth

Authors - Chunming Gu
humanWB

Int J Mol Sci

Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y

Authors - Ilaria Nigro
mouseWB

Front Cell Dev Biol

Transmembrane Protein Ttyh1 Maintains the Quiescence of Neural Stem Cells Through Ca2+/NFATc3 Signaling.

Authors - Yuan Cao
mouseWB

J Innate Immun

Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery.

Authors - Yunzhi Liu
mouseWB

Cell Calcium

Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.

Authors - Yu Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (01-19-2023)

The Ab worked well for WB. 20ug protein loaded. Probed overnight at 4 deg.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 in 5% NFDM
ORAI1 Antibody Western Blot validation (1:2000 in 5% NFDM dilution) in (Cat no:66223-1-Ig)