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ORAI1 Polyclonal antibody

ORAI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14442-1-AP

Synonyms

CRACM1, ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ORAI1 Polyclonal antibody (14442-1-AP)

    WB analysis using 14442-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14442-1-AP (ORAI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman peripheral blood platelets, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IPSee 1 publications below

Product Information

14442-1-AP targets ORAI1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ORAI calcium release-activated calcium modulator 1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 30-35 kDa, 45-55 kDa
GenBank Accession NumberNM_032790
Gene Symbol ORAI1
Gene ID (NCBI) 84876
RRIDAB_2157435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a ~35 kDa plasma membrane protein with 4 transmembrane domains. ORAI1 is a complex glycosylated protein, it exists in several molecular weight forms: a theoretical molecular weight of 32.7 kDa of the unmodified protein and two major glycosylated proteins of ~43 kDa and ~50 kDa (PMID: 22318387). Human patients with a severe combined immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene. This antibody may be used to block the ion currents mediated by Orai1.

Protocols

Product Specific Protocols
WB protocol for ORAI1 antibody 14442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP

BMJ Open Diabetes Res Care

Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.

Authors - Yuan Wei
View Article