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oncostatin M Polyclonal antibody

oncostatin M Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27792-1-AP

Synonyms

oncostatin M, OSM

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using oncostatin M Polyclonal antibody (27792-1-AP)

    WB analysis of MCF-7 using 27792-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27792-1-AP (OSM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using oncostatin M Polyclonal antibody (27792-1-AP)

    WB analysis of Jurkat using 27792-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27792-1-AP (OSM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using oncostatin M Polyclonal antibody (27792-1-AP)

    WB analysis of rat testis using 27792-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 27792-1-AP (OSM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using oncostatin M Polyclonal antibody (27792-1-AP)

    IHC staining of human colon cancer using 27792-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27792-1-AP (OSM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using oncostatin M Polyclonal antibody (27792-1-AP)

    IHC staining of human colon cancer using 27792-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27792-1-AP (OSM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using oncostatin M Polyclonal antibody (27792-1-AP)

    IHC staining of human cervical cancer using 27792-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27792-1-AP (OSM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using oncostatin M Polyclonal antibody (27792-1-AP)

    IHC staining of human cervical cancer using 27792-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27792-1-AP (OSM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells, rat testis tissue
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27792-1-AP targets oncostatin M in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen oncostatin M fusion protein Ag27093
Full Name oncostatin M
Calculated Molecular Weight 252 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC011589
Gene Symbol OSM
Gene ID (NCBI) 5008
RRIDAB_2880974
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for oncostatin M antibody 27792-1-APDownload protocol
IHC protocol for oncostatin M antibody 27792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Jpn J Ophthalmol

Role of oncostatin M in the pathogenesis of vernal keratoconjunctivitis: focus on tissue remodeling.

Authors - Keitaro Mashimo
View Article
mouseIHC

Int J Radiat Oncol Biol Phys

Oncostatin M/Oncostatin M Receptor Signal Induces Radiation-Induced Heart Fibrosis by Regulating SMAD4 in Fibroblast

Authors - Peng Xu
View Article