Osteopontin Polyclonal antibody

Osteopontin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25715-1-AP

Synonyms

BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293, rat kidney tissue, HEK-293 cells, C2C12 cells
Positive IHC detected inhuman stomach cancer tissue, human kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25715-1-AP is Osteopontin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteopontin fusion protein Ag22588
Full Name secreted phosphoprotein 1
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC007016
Gene Symbol SPP1
Gene ID (NCBI) 6696
RRIDAB_2880206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).

Protocols

Product Specific Protocols
WB protocol for Osteopontin antibody 25715-1-APDownload protocol
IHC protocol for Osteopontin antibody 25715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nat Neurosci

Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2.

Authors - Xiang Zhou
mouseIHC

Sci Adv

Diversified transcriptional responses of myeloid and glial cells in spinal cord injury shaped by HDAC3 activity.

Authors - Shalaka Wahane
mouseIHC

Part Fibre Toxicol

Aryl hydrocarbon receptor activation-mediated vascular toxicity of ambient fine particulate matter: contribution of polycyclic aromatic hydrocarbons and osteopontin as a biomarker.

Authors - Chia-Chi Ho
mouseWB

Cell Mol Life Sci

Metastasis suppressor 1 controls osteoblast differentiation and bone homeostasis through regulating Src-Wnt/β-catenin signaling.

Authors - Meng Chen
human,mouseWB

Clin Transl Immunology

Deficient DNASE1L3 facilitates neutrophil extracellular traps-induced invasion via cyclic GMP-AMP synthase and the non-canonical NF-κB pathway in diabetic hepatocellular carcinoma.

Authors - Na Li
mouseWB

Stem Cells

High-mobility group AT-Hook 1 mediates the role of nuclear factor I/X in osteogenic differentiation through activating canonical Wnt signaling.

Authors - Xiaowen Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Eli (Verified Customer) (01-31-2020)

Loaded 20 (lanes 2 and 3) , 10 (lanes 4 and 5), 5 (lanes 6 and 7), and 2.5 (lanes 8 and 9) ug of cell lysates to the lanes listed.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Osteogenically Differentiated hMSCs
Osteopontin Antibody Western Blot, validation (1:1000 dilution) in Osteogenically Differentiated hMSCs (Cat no:25715-1-AP)