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  • Featured Product
  • KD/KO Validated

P4HA1 Monoclonal antibody

P4HA1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H10A6

Cat no : 66101-1-Ig

Synonyms

4 PH alpha 1, P4HA, P4HA1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis using 66101-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of PC-3 using 66101-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of HEK-293 using 66101-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of HSC-T6 using 66101-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of ROS1728 using 66101-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of Neuro-2a using 66101-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of 4T1 using 66101-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of rat liver using 66101-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of NIH/3T3 using 66101-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using P4HA1 Monoclonal antibody (66101-1-Ig)

    IHC staining of human breast cancer using 66101-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using P4HA1 Monoclonal antibody (66101-1-Ig)

    IHC staining of human breast cancer using 66101-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using P4HA1 Monoclonal antibody (66101-1-Ig)

    IHC staining of human testis using 66101-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using P4HA1 Monoclonal antibody (66101-1-Ig)

    IHC staining of human testis using 66101-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    IF Staining of PC-3 using 66101-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed PC-3 cells using 66101-1-Ig(P4HA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, HeLa cells, HSC-T6 cells, ROS1728 cells, Neuro-2a cells, 4T1 cells, rat liver tissue, NIH/3T3 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66101-1-Ig is P4HA1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P4HA1 fusion protein Ag17867
Full Name prolyl 4-hydroxylase, alpha polypeptide I
Calculated Molecular Weight 534 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC034998
Gene Symbol P4HA1
Gene ID (NCBI) 5033
RRIDAB_2881500
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.

Protocols

Product Specific Protocols
WB protocol for P4HA1 antibody 66101-1-IgDownload protocol
IHC protocol for P4HA1 antibody 66101-1-IgDownload protocol
IF protocol for P4HA1 antibody 66101-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Oncol

Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.

Authors - Johanna Eriksson
  • KD Validated
View Article
humanWB

Front Oncol

P4HA1 Regulates CD31 via COL6A1 in the Transition of Glioblastoma Stem-Like Cells to Tumor Endothelioid Cells.

Authors - Xiangming Han
  • KD Validated
View Article
humanWB

Front Mol Biosci

Identification of Prognostic Biomarkers in Patients With Malignant Rhabdoid Tumor of the Kidney Based on mTORC1 Signaling Pathway-Related Genes.

Authors - Chenghao Zhanghuang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sumreet Singh (Verified Customer) (11-30-2018)

This antibody works very well for our data analysis and serves our purpose. It does not give any background and works great. Please send gift card from Amazon to this address:Accounts Payable Specialist Modern Meadow, Inc.340 Kingsland Street, Building 102Nutley, NJ 07110

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: Purified protein