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PABPC4 Polyclonal antibody

Name: PABPC4 Polyclonal antibody
Brand: Proteintech
SKU: 14960-1-AP

PABPC4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14960-1-AP

Synonyms

Activated platelet protein 1, APP 1, APP1, iPABP, PABP 4, PABP4, PABPC4, Poly(A) binding protein 4

Validation Data Gallery

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AllWBIPIHCIF

WB analysis using 14960-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14960-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 14960-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14960-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 14960-1-AP
IP result of anti-PABPC4 (IP:14960-1-AP, 4ug; Detection:14960-1-AP 1:500) with mouse heart tissue lysate 4000ug.
IHC staining of human gliomas using 14960-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 14960-1-AP (PABPC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human gliomas using 14960-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 14960-1-AP (PABPC4 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 14960-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PABPC4 antibody (14960-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 14960-1-AP
Immunofluorescent analysis of Hela cells, using PABPC4 antibody 14960-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 14960-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, mouse heart tissue, human brain tissue, Jurkat cells
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

14960-1-AP targets PABPC4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PABPC4 fusion protein Ag6813
Full Name	poly(A) binding protein, cytoplasmic 4 (inducible form)
Calculated Molecular Weight	70 kDa
Observed Molecular Weight	70-71 kDa
GenBank Accession Number	BC065540
Gene Symbol	PABPC4
Gene ID (NCBI)	8761
RRID	AB_2156897
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PABPC4 antibody 14960-1-AP	Download protocol
IHC protocol for PABPC4 antibody 14960-1-AP	Download protocol
IF protocol for PABPC4 antibody 14960-1-AP	Download protocol
IP protocol for PABPC4 antibody 14960-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Nucleic Acids Res Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Authors - Evan P Booy View Article
human	WB	J Immunol HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins. Authors - Adi Reches KD Validated View Article
human	WB	BMC Pregnancy Childbirth Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics. Authors - Xiaotong Sun View Article
human	WB	PLoS Pathog Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes Authors - Emily Yang View Article
mouse	IF	Cell Rep The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation Authors - Nicolas Curdy View Article
human	WB	Nat Cell Biol An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus Authors - Albertas Navickas View Article

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Research Area

PABPC4 Polyclonal antibody

PABPC4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 14960-1-AP

WB analysis of mouse heart using 14960-1-AP

WB analysis of human brain using 14960-1-AP

WB analysis of Jurkat using 14960-1-AP

IP experiment of mouse heart using 14960-1-AP

IHC staining of human gliomas using 14960-1-AP

IHC staining of human gliomas using 14960-1-AP

IF Staining of HepG2 using 14960-1-AP

IF Staining of HeLa using 14960-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.

Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics.

Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation

An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus