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PACS2-Specific Polyclonal antibody
PACS2-Specific Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 19508-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 15 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
| IP | See 2 publications below |
Product Information
19508-1-AP targets PACS2-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | phosphofurin acidic cluster sorting protein 2 |
| Calculated Molecular Weight | 98 kDa |
| Observed Molecular Weight | 116 kDa |
| GenBank Accession Number | NM_015197 |
| Gene Symbol | PACS2 |
| Gene ID (NCBI) | 23241 |
| RRID | AB_10643387 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PACS2, also named PACS1L or KIAA0602, is one of PACS (phosphofurin acidic cluster sorting) proteins which are one such family of multifunctional membrane traffic regulators that mediate organ homoeostasis and have important roles in diverse pathologies and disease states. PACS2 is a multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, ER homeostasis, and apoptosis. PACS2 may be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PACS2-Specific antibody 19508-1-AP | Download protocol |
| IHC protocol for PACS2-Specific antibody 19508-1-AP | Download protocol |
| IF protocol for PACS2-Specific antibody 19508-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Circulation Hyperglycemia-Driven Inhibition of AMP-Activated Protein Kinase α2 Induces Diabetic Cardiomyopathy by Promoting Mitochondria-Associated Endoplasmic Reticulum Membranes In Vivo. | ||
EMBO J MITOL prevents ER stress-induced apoptosis by IRE1α ubiquitylation at ER-mitochondria contact sites.
| ||
Nat Commun The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17.
| ||
EMBO J MITOL prevents ER stress-induced apoptosis by IRE1α ubiquitylation at ER-mitochondria contact sites.
| ||
Diabetes PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy. |
