Paladin belongs to the myotilin/palladin family. Palladin is a widely expressed protein found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It is a cytoskeletal protein required for organization of normal actin cytoskeleton. It functions in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. Palladin has multiple transcription isoforms, specialized for different functions. This antibody, generated against N-terminal peptide of palladin, recognizes 200 kD, 140 kD, and 65 kD isoforms of this protein.