PAM Polyclonal antibody
PAM Polyclonal Antibody for ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB,ELISA
Conjugate
Unconjugated
Cat no : 15464-1-AP
Synonyms
Validation Data Gallery
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15464-1-AP targets PAM in WB,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PAM fusion protein Ag7762 |
| Full Name | peptidylglycine alpha-amidating monooxygenase |
| Calculated Molecular Weight | 108 kDa |
| GenBank Accession Number | BC018127 |
| Gene Symbol | PAM |
| Gene ID (NCBI) | 5066 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAM(Peptidyl-glycine alpha-amidating monooxygenase) is a multifunctional protein containing 2 enzymes,PHM and PAL. The PAM protein containing the peptide encoded by exon A (Type B mRNA) is cleaved to form 41-and 43-kDa proteins,and the 75kDa PAM proteins is determined by lacking antigenic exon B(TypeA)(PMID:1740449).The substrate specificity of PAM is broad, and production of all a-amidated peptides may be dependent upon proteins encoded by the single copy PAM gene(PMID:8436961).It has 6 isoforms produced by alternative splicing.
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem Proteomic profiling of androgen-independent prostate cancer cell lines reveals a role for protein S during the development of high grade and castration-resistant prostate cancer. |
