PAOX Polyclonal antibody
PAOX Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 18972-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse pancreas tissue, human stomach tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
18972-1-AP targets PAOX in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PAOX fusion protein Ag5777 |
| Full Name | polyamine oxidase (exo-N4-amino) |
| Calculated Molecular Weight | 511 aa, 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC032778 |
| Gene Symbol | PAOX |
| Gene ID (NCBI) | 196743 |
| RRID | AB_2159196 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAOX, also known as PAO, belongs to the flavin monoamine oxidase family. PAOX is a FAD-dependent amine oxidase, which is constitutively expressed in nearly all tissues of the vertebrate organism. PAOX catalyzes the oxidation of N1-acetylspermine to spermidine and thus involved in the polyamine back-conversion. PAOX can also oxidize N1-acetylspermidine to putrescine. PAOX has 4 isoforms with the molecular weights of 7, 35, 52 and 56 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAOX antibody 18972-1-AP | Download protocol |
| IHC protocol for PAOX antibody 18972-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Regulation of Spermine Oxidase through Hypoxia-Inducible Factor-1α Signaling in Retinal Glial Cells under Hypoxic Conditions. | ||
Biol Reprod Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period. | ||
PNAS Nexus Acidic extracellular pH drives accumulation of N1-acetylspermidine and recruitment of protumor neutrophils |
