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PARD3 Monoclonal antibody

PARD3 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D2B2

Cat no : 66968-1-Ig

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    WB analysis of MCF-7 using 66968-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    WB analysis of HepG2 using 66968-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PARD3 Monoclonal antibody (66968-1-Ig)

    WB analysis of HeLa using 66968-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    WB analysis of HEK-293 using 66968-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    WB analysis of A431 using 66968-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    IF Staining of MCF-7 using 66968-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PARD3 antibody (66968-1-Ig, Clone: 1D2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    FC experiment of MCF-7 using 66968-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PARD3 (66968-1-Ig, Clone:1D2B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, A431 cells, HepG2 cells
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66968-1-Ig targets PARD3 in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PARD3 fusion protein Ag7656
Full Name par-3 partitioning defective 3 homolog (C. elegans)
Calculated Molecular Weight 151 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC011711
Gene Symbol PARD3
Gene ID (NCBI) 56288
RRIDAB_2882290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts.

Protocols

Product Specific Protocols
WB protocol for PARD3 antibody 66968-1-IgDownload protocol
IF protocol for PARD3 antibody 66968-1-IgDownload protocol
FC protocol for PARD3 antibody 66968-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols