• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PARN Polyclonal antibody

PARN Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13799-1-AP

Synonyms

DAN, Deadenylating nuclease, Deadenylation nuclease, PARN

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PARN Polyclonal antibody (13799-1-AP)

    WB analysis using 13799-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13799-1-AP (PARN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human cerebellum tissue using PARN Polyclonal antibody (13799-1-AP)

    WB analysis of human cerebellum using 13799-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13799-1-AP (PARN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using PARN Polyclonal antibody (13799-1-AP)

    IHC staining of human endometrial cancer using 13799-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13799-1-AP (PARN antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using PARN Polyclonal antibody (13799-1-AP)

    IHC staining of human endometrial cancer using 13799-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13799-1-AP (PARN antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, human cerebellum tissue, Jurkat cell, MDA-MB-453s cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13799-1-AP targets PARN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARN fusion protein Ag4774
Full Name poly(A)-specific ribonuclease (deadenylation nuclease)
Calculated Molecular Weight 639 aa, 73 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC050029
Gene Symbol PARN
Gene ID (NCBI) 5073
RRIDAB_2160430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PARN antibody 13799-1-APDownload protocol
IHC protocol for PARN antibody 13799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Hum Mol Genet

CGG repeats in RNA modulate expression of TDP-43 in mouse and fly models of fragile X tremor ataxia syndrome.

Authors - Jocelyn N Galloway
View Article
humanIF

Neurobiol Dis

Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes.

Authors - Rebecca L Kow
View Article
mouseWB

Cell Rep

YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways.

Authors - Jiadong Liu
View Article
humanWB

Nucleic Acids Res

Molecular mechanism of human ISG20L2 for the ITS1 cleavage in the processing of 18S precursor ribosomal RNA

Authors - Yinliang Ma
View Article