PARP1 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66520-1-Ig |CloneNo.: 1D7D4

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Con: 75 μg/150 μl

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Species specificity:
Human, mouse, rat

Positive WB detected in:
Jurkat cells,RAW 264.7 cells, HSCT6 cells, HeLa cells

Positive IHC detected in:
human lung cancer tissue

Recommended dilution:
WB : 1:10000-1:100000
IHC : 1:100-1:1200

Product Information


Source:
Mouse

Purification method:
Protein G purification

Isotype:
IgG1

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
poly (ADP-ribose) polymerase 1

Calculated molecular weight:
1014aa,113 kDa

Observed molecular weight:
113-116, 85-89 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PARP1

Synonyms
ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL
Background

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kD. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.


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