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PARP3 Polyclonal antibody

PARP3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11289-1-AP

Synonyms

ADPRT 3, ADPRT3, ADPRTL2, ADPRTL3, hPARP 3, IRT1, PADPRT 3, PARP 3, PARP3, Poly [ADP ribose] polymerase 3, Poly[ADP ribose] synthase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARP3 Polyclonal antibody (11289-1-AP)

    WB analysis using 11289-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using PARP3 Polyclonal antibody (11289-1-AP)

    WB analysis of human kidney using 11289-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using PARP3 Polyclonal antibody (11289-1-AP)

    WB analysis of rat heart using 11289-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using PARP3 Polyclonal antibody (11289-1-AP)

    WB analysis of human heart using 11289-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PARP3 Polyclonal antibody (11289-1-AP)

    IHC staining of human pancreas cancer using 11289-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11289-1-AP (PARP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PARP3 Polyclonal antibody (11289-1-AP)

    IHC staining of human breast cancer using 11289-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PARP3 Polyclonal antibody (11289-1-AP)

    IHC staining of human renal cell carcinoma using 11289-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PARP3 Polyclonal antibody (11289-1-AP)

    IHC staining of human breast cancer using 11289-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PARP3 Polyclonal antibody (11289-1-AP)

    IF Staining of HeLa using 11289-1-AP

    Immunofluorescent analysis of Hela cells, using PARP3 antibody 11289-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, human kidney tissue, rat heart tissue, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11289-1-AP targets PARP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARP3 fusion protein Ag1844
Full Name poly (ADP-ribose) polymerase family, member 3
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-62 kDa
GenBank Accession NumberBC014260
Gene Symbol PARP3
Gene ID (NCBI) 10039
RRIDAB_2283392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PARP3 antibody 11289-1-APDownload protocol
IHC protocol for PARP3 antibody 11289-1-APDownload protocol
IF protocol for PARP3 antibody 11289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Unrestrained poly-ADP-ribosylation provides insights into chromatin regulation and human disease.

Authors - Evgeniia Prokhorova
View Article
humanWB

Cell Chem Biol

Allosteric regulation of DNA binding and target residence time drive the cytotoxicity of phthalazinone-based PARP-1 inhibitors

Authors - Moriah R Arnold
  • KD Validated
View Article