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PCBD1 Polyclonal antibody

PCBD1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15702-1-AP

Synonyms

DCOH, Dimerization cofactor of HNF1, PCBD, PCBD1, PCD, PHS

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    WB analysis of mouse pancreas using 15702-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15702-1-AP (PCBD1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    WB analysis of mouse pancreas using 15702-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15702-1-AP (PCBD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of mouse kidney using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of mouse kidney using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human ovary tumor using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15702-1-AP (PCBD1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human ovary tumor using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15702-1-AP (PCBD1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCBD1 Polyclonal antibody (15702-1-AP)

    IF Staining of HeLa using 15702-1-AP

    Immunofluorescent analysis of Hela cells, using PCBD1 antibody 15702-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15702-1-AP targets PCBD1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCBD1 fusion protein Ag8331
Full Name pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
Calculated Molecular Weight 104 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC006324
Gene Symbol PCBD1
Gene ID (NCBI) 5092
RRIDAB_2267945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PCBD1 antibody 15702-1-APDownload protocol
IHC protocol for PCBD1 antibody 15702-1-APDownload protocol
IF protocol for PCBD1 antibody 15702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols