PCK2 Monoclonal antibody

PCK2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B3F1

Cat no : 67676-1-Ig

Synonyms

PCK2, PEPCK, PEPCK M, PEPCK2



Tested Applications

Positive WB detected inHuH-7 cells, rat liver tissue, pig liver tissue, rabbit liver tissue, HepG2 cells, LNCaP cells, K562 cells, Neuro-2a cells, HSC-T6 cells
Positive IHC detected inhuman lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67676-1-Ig targets PCK2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCK2 fusion protein Ag7021
Full Name phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Calculated Molecular Weight 71 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC001454
Gene Symbol PCK2
Gene ID (NCBI) 5106
RRIDAB_2882871
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.

Protocols

Product Specific Protocols
WB protocol for PCK2 antibody 67676-1-IgDownload protocol
IHC protocol for PCK2 antibody 67676-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Food Funct

Black bean husk and black rice anthocyanin extracts modulated gut microbiota and serum metabolites for improvement in type 2 diabetic rats.

Authors - Mubai Sun
human,mouseWB

Front Pharmacol

Berberrubine, a Main Metabolite of Berberine, Alleviates Non-Alcoholic Fatty Liver Disease via Modulating Glucose and Lipid Metabolism and Restoring Gut Microbiota

Authors - Sa Yang