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PCSK7 Polyclonal antibody

PCSK7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12044-1-AP

Synonyms

hPC8, LPC, Lymphoma proprotein convertase, PC7, PC8, PCSK7, Prohormone convertase 7, Proprotein convertase 7, Proprotein convertase 8, SPC7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCSK7 Polyclonal antibody (12044-1-AP)

    WB analysis using 12044-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12044-1-AP (PCSK7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PCSK7 Polyclonal antibody (12044-1-AP)

    IHC staining of human colon cancer using 12044-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12044-1-AP (PCSK7 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PCSK7 Polyclonal antibody (12044-1-AP)

    IHC staining of human lymphoma using 12044-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12044-1-AP (PCSK7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PCSK7 Polyclonal antibody (12044-1-AP)

    IHC staining of human lymphoma using 12044-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12044-1-AP (PCSK7 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

12044-1-AP targets PCSK7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCSK7 fusion protein Ag2674
Full Name proprotein convertase subtilisin/kexin type 7
Calculated Molecular Weight 785 aa, 86 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC010696
Gene Symbol PCSK7
Gene ID (NCBI) 9159
RRIDAB_2877820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCSK7(Proprotein convertase subtilisin/kexin type 7) is also named as LPC, PC7, PC8, SPC7 and belongs to the peptidase S8 family. It has ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RXXX[KR]R consensus motif. The full length protein has 4 glycosylation sites and it can be N-glycosylated.

Protocols

Product Specific Protocols
WB protocol for PCSK7 antibody 12044-1-APDownload protocol
IHC protocol for PCSK7 antibody 12044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Pharmacol

Therapeutic potential of the Proprotein Convertase Subtilisin/Kexin family in vascular disease

Authors - Bianca E. Suur
View Article