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  • KD/KO Validated

PDCD5 Polyclonal antibody

PDCD5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12456-1-AP

Synonyms

PDCD5, programmed cell death 5, Protein TFAR19, TFAR19



Tested Applications

Positive WB detected inhuman heart tissue, PC-3 cells, human brain tissue, A549 cells
Positive IHC detected inhuman endometrial cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12456-1-AP targets PDCD5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDCD5 fusion protein Ag3122
Full Name programmed cell death 5
Calculated Molecular Weight 125 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC015519
Gene Symbol PDCD5
Gene ID (NCBI) 9141
RRIDAB_2162450
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDCD5, also called TFAR19 (TF1 cell apoptosis-related gene 19), was first identified as a gene up-regulated in TF-1 cells under-going apoptosis (PMID: 9920759). PDCD5 can promote pro-grammed cell death in different cell types in response to various stimuli and also enhance TAJ/TROY-induced paraptosis-like cell death (PMID: 15020679). During apoptosis, PDCD5 is rapidly upregulated and translocates from the cytoplasm to nucleus (PMID: 11741587). PDCD5 is a positive regulator of Tip60 and also has a potential ability to interact with p53 (PMID: 22914926, PMID: 19308289). Recent studies have also revealed that PDCD5 may be a suppressor gene and expressed at lower levels in many cancers, including hepatocellular carcinoma, breast cancer, gastric cancer, cervical cancer, lung cancer, astrocytic gliomas, and in leukemia.

Protocols

Product Specific Protocols
WB protocol for PDCD5 antibody 12456-1-APDownload protocol
IHC protocol for PDCD5 antibody 12456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC, WB

Nat Commun

Club cell-specific role of programmed cell death 5 in pulmonary fibrosis.

Authors - Soo-Yeon Park
WB

Nat Commun

Programmed cell death 5 mediates HDAC3 decay to promote genotoxic stress response.

Authors - Hyo-Kyoung Choi
  • KD Validated
humanWB

J Nanobiotechnology

Multifunctional photodynamic/photothermal nano-agents for the treatment of oral leukoplakia.

Authors - Lin Lin
humanWB

J Cell Physiol

Thyroid stimulating hormone aggravates diabetic retinopathy through the mitochondrial apoptotic pathway.

Authors - Dong Lin
humanWB

Sci Rep

Protein serine/threonine phosphatase PPEF-1 suppresses genotoxic stress response via dephosphorylation of PDCD5.

Authors - Soo-Yeon Park
humanWB, IF

Cancer Lett

DNAJB1 destabilizes PDCD5 to suppress p53-mediated apoptosis.

Authors - Xiandan Cui