PDGFR alpha Monoclonal antibody
PDGFR alpha Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
WB, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2C11B8
Cat no : 60045-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, HT-1080 cells, HUVEC cells, U-251 cells |
| Positive FC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Flow Cytometry (FC) | FC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 3 publications below |
Product Information
60045-1-Ig targets PDGFR alpha in WB, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PDGFR alpha fusion protein Ag1385 |
| Full Name | platelet-derived growth factor receptor, alpha polypeptide |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 100-120 kDa, 140-160 kDa |
| GenBank Accession Number | BC015186 |
| Gene Symbol | PDGFRA |
| Gene ID (NCBI) | 5156 |
| RRID | AB_2162343 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival and chemotaxis. Mutations in PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDGFR alpha antibody 60045-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine. | ||
Development The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition | ||
Development The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition. | ||
Metabolism The vitamin D receptor regulates miR-140-5p and targets the MAPK pathway in bone development. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tom (Verified Customer) (11-25-2021) | HEK293 lysate - some non-specific bands below real band observed.
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