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PDGFRL Polyclonal antibody

PDGFRL Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 16217-1-AP

Synonyms

PDGFR like protein, PDGFRL, PDGRL, PRLTS

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using PDGFRL Polyclonal antibody (16217-1-AP)

    WB analysis of L02 using 16217-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16217-1-AP (PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PDGFRL Polyclonal antibody (16217-1-AP)

    WB analysis of mouse liver using 16217-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16217-1-AP (PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PDGFRL Polyclonal antibody (16217-1-AP)

    IHC staining of human liver cancer using 16217-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PDGFRL Polyclonal antibody (16217-1-AP)

    IHC staining of human liver cancer using 16217-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PDGFRL Polyclonal antibody (16217-1-AP)

    IHC staining of mouse liver using 16217-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PDGFRL Polyclonal antibody (16217-1-AP)

    IHC staining of mouse liver using 16217-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using PDGFRL Polyclonal antibody (16217-1-AP)

    IF Staining of L02 using 16217-1-AP

    Immunofluorescent analysis of L02 cells using 16217-1-AP (PDGFRL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using PDGFRL Polyclonal antibody (16217-1-AP)

    FC experiment of HepG2 using 16217-1-AP

    1X10^6 HepG2 cells were stained with .2ug PDGFRL antibody (16217-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inL02 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16217-1-AP targets PDGFRL in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDGFRL fusion protein Ag9218
Full Name platelet-derived growth factor receptor-like
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC010927
Gene Symbol PDGFRL
Gene ID (NCBI) 5157
RRIDAB_2878230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDGFRL antibody 16217-1-APDownload protocol
IHC protocol for PDGFRL antibody 16217-1-APDownload protocol
IF protocol for PDGFRL antibody 16217-1-APDownload protocol
FC protocol for PDGFRL antibody 16217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols