PDI Monoclonal antibody
PDI Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig, mouse, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2E6A11
Cat no : 66422-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, rat brain tissue, HepG2 cells, L02 cells, COLO 320 cells |
| Positive IHC detected in | human liver tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 12 publications below |
Product Information
66422-1-Ig targets PDI in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse, rat samples.
| Tested Reactivity | human, pig, mouse, rat |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PDI fusion protein Ag1747 |
| Full Name | prolyl 4-hydroxylase, beta polypeptide |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC014504 |
| Gene Symbol | P4HB |
| Gene ID (NCBI) | 5034 |
| RRID | AB_2881794 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDI antibody 66422-1-Ig | Download protocol |
| IHC protocol for PDI antibody 66422-1-Ig | Download protocol |
| IF protocol for PDI antibody 66422-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Cell Res SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization | ||
J Cell Sci A general role for TANGO1, encoded by MIA3, in secretory pathway organization and function | ||
Mol Pharm Highly Efficient Method for Intracellular Delivery of Proteins Mediated by Cholera Toxin-Induced Protein Internalization. | ||
Cell Signal HRD1-mediated PTEN degradation promotes cell proliferation and hepatocellular carcinoma progression. | ||
Biochim Biophys Acta Gen Subj Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels | ||
Mol Med Rep Sigma‑1 receptor overexpression promotes proliferation and ameliorates cell apoptosis in β‑cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tom (Verified Customer) (12-15-2020) | 10ug total protein of HEK293T lysate loaded. Membrane blocked in 5% BSA. Antibody (1:5,000) incubated overnight in block at 4 degrees. Anti-mouse HRP used at 1 in 10,000 to detect band.
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