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Podoplanin Polyclonal antibody

Podoplanin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22099-1-AP

Synonyms

AGGRUS, D2 40, D2 40,M2A, D240, Glycoprotein 36, GP36, Gp38, GP40, HT1A 1, M2A, OTS8, PA2.26, PA2.26 antigen, PDPN, Podoplanin, PSEC0025, T1 alpha, T1A, T1A 2

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    WB analysis of human brain using 22099-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    WB analysis of human brain using 22099-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    WB analysis of human placenta using 22099-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human appendicitis using 22099-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human appendicitis using 22099-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human lymphoma using 22099-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human lymphoma using 22099-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue, human placenta tissue
Positive IHC detected inhuman appendicitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22099-1-AP is Podoplanin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Podoplanin fusion protein Ag17307
Full Name podoplanin
Calculated Molecular Weight 238 aa, 25 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC022812
Gene Symbol PDPN
Gene ID (NCBI) 10630
RRIDAB_2878995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Podoplanin antibody 22099-1-APDownload protocol
IHC protocol for Podoplanin antibody 22099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols