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PDXDC1 Polyclonal antibody

PDXDC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21021-1-AP

Synonyms

KIAA0251, LP8165, PDXDC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDXDC1 Polyclonal antibody (21021-1-AP)

    WB analysis using 21021-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDXDC1 Polyclonal antibody (21021-1-AP)

    WB analysis using 21021-1-AP

    mouse testis lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PDXDC1 Polyclonal antibody (21021-1-AP)

    IP experiment of HepG2 using 21021-1-AP

    IP result of anti-PDXDC1 (IP:21021-1-AP, 4ug; Detection:21021-1-AP 1:500) with HepG2 cells lysate 1680 ug.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using PDXDC1 Polyclonal antibody (21021-1-AP)

    IHC staining of human hepatocirrhosis using 21021-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using PDXDC1 Polyclonal antibody (21021-1-AP)

    IHC staining of human hepatocirrhosis using 21021-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using PDXDC1 Polyclonal antibody (21021-1-AP)

    IHC staining of human testis using 21021-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PDXDC1 Polyclonal antibody (21021-1-AP)

    IHC staining of human testis using 21021-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDXDC1 Polyclonal antibody (21021-1-AP)

    IF Staining of HepG2 using 21021-1-AP

    Immunofluorescent analysis of HepG2 cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PDXDC1 Polyclonal antibody (21021-1-AP)

    IF Staining of HeLa using 21021-1-AP

    Immunofluorescent analysis of Hela cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue, HeLa cells, SGC-7901 cells, rat testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21021-1-AP is PDXDC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDXDC1 fusion protein Ag15283
Full Name pyridoxal-dependent decarboxylase domain containing 1
Calculated Molecular Weight 788 aa, 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC033748
Gene Symbol PDXDC1
Gene ID (NCBI) 23042
RRIDAB_10695768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDXDC1 antibody 21021-1-APDownload protocol
IHC protocol for PDXDC1 antibody 21021-1-APDownload protocol
IF protocol for PDXDC1 antibody 21021-1-APDownload protocol
IP protocol for PDXDC1 antibody 21021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Mol Med (Berl)

Identification of PDXDC1 as a novel pleiotropic susceptibility locus shared between lumbar spine bone mineral density and birth weight.

Authors - Yu-Qian Song
View Article
mouseWB

Cell Mol Life Sci

Protease-independent control of parthanatos by HtrA2/Omi

Authors - Jonas Weiß
View Article