• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PDZK1 Polyclonal antibody

PDZK1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10507-2-AP

Synonyms

CAP70, CLAMP, NaPi Cap1, NHERF 3, NHERF3, PDZ domain containing 1, PDZD1, PDZK1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse kidney tissue using PDZK1 Polyclonal antibody (10507-2-AP)

    WB analysis of mouse kidney using 10507-2-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10507-2-AP (PDZK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PDZK1 Polyclonal antibody (10507-2-AP)

    WB analysis of HEK-293 using 10507-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10507-2-AP (PDZK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PDZK1 Polyclonal antibody (10507-2-AP)

    IP experiment of HEK-293 using 10507-2-AP

    IP result of anti-PDZK1 (IP:10507-2-AP, 3ug; Detection:10507-2-AP 1:500) with HEK-293 cells lysate 3200ug.

Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10507-2-AP targets PDZK1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDZK1 fusion protein Ag0787
Full Name PDZ domain containing 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 63-70 kDa
GenBank Accession NumberBC006496
Gene Symbol PDZK1
Gene ID (NCBI) 5174
RRIDAB_2299495
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDZK1 antibody 10507-2-APDownload protocol
IP protocol for PDZK1 antibody 10507-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.

Authors - Yi Huo
View Article
humanIHC

Mol Med

PDZK1 Is a Novel Factor in Breast Cancer That Is Indirectly Regulated by Estrogen through IGF-1R and Promotes Estrogen-Mediated Growth.

Authors - Kim Hogyoung H
View Article
humanIHC

Mol Med

Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37.

Authors - Hogyoung Kim
View Article