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  • Featured Product
  • KD/KO Validated

PEA15 Polyclonal antibody

PEA15 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21446-1-AP

Synonyms

HMAT1, HUMMAT1H, MAT1, MAT1H, PEA 15, PEA15, PED

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using PEA15 Polyclonal antibody (21446-1-AP)

    WB analysis of fetal human brain using 21446-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21446-1-AP (PEA15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PEA15 Polyclonal antibody (21446-1-AP)

    IHC staining of mouse brain using 21446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21446-1-AP (PEA15 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PEA15 Polyclonal antibody (21446-1-AP)

    IHC staining of mouse brain using 21446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21446-1-AP (PEA15 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PEA15 Polyclonal antibody (21446-1-AP)

    IHC staining of mouse brain using 21446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21446-1-AP (PEA15 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected infetal human brain tissue
Positive IHC detected inmouse brain tissue, human heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21446-1-AP is PEA15 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEA15 fusion protein Ag13782
Full Name phosphoprotein enriched in astrocytes 15
Calculated Molecular Weight 130 aa, 15 kDa
Observed Molecular Weight 15-18 kDa
GenBank Accession NumberBC002426
Gene Symbol PEA15
Gene ID (NCBI) 8682
RRIDAB_2878858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PEA15 antibody 21446-1-APDownload protocol
IHC protocol for PEA15 antibody 21446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cancer

The microRNA212 regulated PEA15 promotes ovarian cancer progression by inhibiting of apoptosis.

Authors - Yonghong Luo
  • KD Validated
View Article