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PEF1 Polyclonal antibody

PEF1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 10151-1-AP

Synonyms

ABP32, PEF1, PEF1A, PEFLIN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PEF1 Polyclonal antibody (10151-1-AP)

    WB analysis using 10151-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10151-1-AP (PEF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PEF1 Polyclonal antibody (10151-1-AP)

    IP experiment of HEK-293 using 10151-1-AP

    IP result of anti-PEF1 (IP:10151-1-AP, 4ug; Detection:10151-1-AP 1:500) with HEK-293 cells lysate 3200ug.

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10151-1-AP targets PEF1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEF1 fusion protein Ag0198
Full Name penta-EF-hand domain containing 1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC002773
Gene Symbol PEF1
Gene ID (NCBI) 553115
RRIDAB_2161168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PEF1 antibody 10151-1-APDownload protocol
IP protocol for PEF1 antibody 10151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Proteome Res

Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma.

Authors - Yang Xiaoli X
View Article