• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

PEX1 Polyclonal antibody

PEX1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13669-1-AP

Synonyms

Peroxin 1, Peroxisome biogenesis factor 1, PEX1, ZWS1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using PEX1 Polyclonal antibody (13669-1-AP)

    WB analysis of Jurkat using 13669-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13669-1-AP (PEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PEX1 Polyclonal antibody (13669-1-AP)

    WB analysis of HeLa using 13669-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13669-1-AP (PEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PEX1 Polyclonal antibody (13669-1-AP)

    IHC staining of human liver cancer using 13669-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PEX1 Polyclonal antibody (13669-1-AP)

    IHC staining of human liver cancer using 13669-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX1 Polyclonal antibody (13669-1-AP)

    IHC staining of human kidney using 13669-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX1 Polyclonal antibody (13669-1-AP)

    IHC staining of human kidney using 13669-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PEX1 Polyclonal antibody (13669-1-AP)

    IF Staining of HepG2 using 13669-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 13669-1-AP (PEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PEX1 Polyclonal antibody (13669-1-AP)

    IF Staining of A431 using 13669-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 13669-1-AP (PEX1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PEX1 Polyclonal antibody (13669-1-AP)

    IF Staining of HeLa using 13669-1-AP

    Immunofluorescent analysis of HeLa cells using 13669-1-AP (PEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, A431 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13669-1-AP targets PEX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX1 fusion protein Ag4623
Full Name peroxisomal biogenesis factor 1
Calculated Molecular Weight 1283 aa, 143 kDa
Observed Molecular Weight 143 kDa
GenBank Accession NumberBC035575
Gene Symbol PEX1
Gene ID (NCBI) 5189
RRIDAB_2162104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PEX1 antibody 13669-1-APDownload protocol
IHC protocol for PEX1 antibody 13669-1-APDownload protocol
IF protocol for PEX1 antibody 13669-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Autophagy

Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.

Authors - Marcus Nordgren
View Article
humanWB,IF

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated
View Article
humanWB

Autophagy

AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.

Authors - Zhenhong Ni
View Article
humanIHC

Eur J Hum Genet

Spectrum of PEX1 and PEX6 variants in Heimler syndrome.

Authors - Claire E L Smith
View Article
humanWB

Mol Ther Methods Clin Dev

AAV-mediated PEX1 gene augmentation improves visual function in the PEX1-Gly844Asp mouse model for mild Zellweger spectrum disorder.

Authors - Catherine Argyriou
View Article
mouseWB,IF

Exp Eye Res

A longitudinal study of retinopathy in the PEX1-Gly844Asp mouse model for mild Zellweger Spectrum Disorder.

Authors - Catherine Argyriou
View Article