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PEX5L Polyclonal antibody

PEX5L Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13084-1-AP

Synonyms

Peroxin 5 related protein, PEX2 related protein, PEX5 like protein, PEX5 related protein, PEX5L, PEX5R, Pex5Rp, PXR2, PXR2B

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PEX5L Polyclonal antibody (13084-1-AP)

    WB analysis of mouse brain using 13084-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13084-1-AP (PEX5L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PEX5L Polyclonal antibody (13084-1-AP)

    WB analysis of mouse brain using 13084-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13084-1-AP (PEX5L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PEX5L Polyclonal antibody (13084-1-AP)

    WB analysis of rat brain using 13084-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 13084-1-AP (PEX5L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PEX5L Polyclonal antibody (13084-1-AP)

    IP experiment of mouse brain using 13084-1-AP

    IP result of anti-PEX5L (IP:13084-1-AP, 4ug; Detection:13084-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PEX5L Polyclonal antibody (13084-1-AP)

    IHC staining of mouse brain using 13084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13084-1-AP (PEX5L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PEX5L Polyclonal antibody (13084-1-AP)

    IHC staining of mouse brain using 13084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13084-1-AP (PEX5L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

13084-1-AP targets PEX5L in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX5L fusion protein Ag3749
Full Name peroxisomal biogenesis factor 5-like
Calculated Molecular Weight 626 aa, 70 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC036183
Gene Symbol PEX5L
Gene ID (NCBI) 51555
RRIDAB_2877914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PEX5L antibody 13084-1-APDownload protocol
IHC protocol for PEX5L antibody 13084-1-APDownload protocol
IP protocol for PEX5L antibody 13084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Mol Psychiatry

Glucocorticoid-glucocorticoid receptor-HCN1 channels reduce neuronal excitability in dorsal hippocampal CA1 neurons.

Authors - Jiwon Kim
View Article