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Pepsinogen II Polyclonal antibody

Pepsinogen II Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16881-1-AP

Synonyms

Gastricsin, Pepsinogen C, Pepsinogen II, PEPSINOGEN, GROUP II, PGⅡ, PGC, Progastricsin, progastricsin (pepsinogen C)

Validation Data Gallery

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  • Western Blot (WB) analysis of human stomach tissue using Pepsinogen II Polyclonal antibody (16881-1-AP)

    WB analysis of human stomach using 16881-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 16881-1-AP (Pepsinogen II antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Pepsinogen II Polyclonal antibody (16881-1-AP)

    WB analysis using 16881-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 16881-1-AP (Pepsinogen II antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Pepsinogen II Polyclonal antibody (16881-1-AP)

    IHC staining of human stomach cancer using 16881-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16881-1-AP (Pepsinogen II antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Pepsinogen II Polyclonal antibody (16881-1-AP)

    IHC staining of human stomach cancer using 16881-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16881-1-AP (Pepsinogen II antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman stomach tissue, mouse stomach tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16881-1-AP targets Pepsinogen II in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Pepsinogen II fusion protein Ag10332
Full Name progastricsin (pepsinogen C)
Calculated Molecular Weight 388 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC073740
Gene Symbol PGC
Gene ID (NCBI) 5225
RRIDAB_10596919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for Pepsinogen II antibody 16881-1-APDownload protocol
IHC protocol for Pepsinogen II antibody 16881-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols