HPGDS Polyclonal antibody

HPGDS Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22522-1-AP

Synonyms

Glutathione S transferase, GST class sigma, GSTS, H PGDS, HPGDS, PGDS, Prostaglandin H2 D isomerase, PTGDS2



Tested Applications

Positive WB detected inrat spleen tissue, mouse small intestine tissue
Positive IHC detected inhuman placenta tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22522-1-AP targets HPGDS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPGDS fusion protein Ag18290
Full Name prostaglandin D2 synthase, hematopoietic
Calculated Molecular Weight 199 aa, 23 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC020734
Gene Symbol PGDS
Gene ID (NCBI) 27306
RRIDAB_2879115
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hematopoietic prostaglandin D synthase (HPGDS) is expressed in human Th2 lymphocytes, antigen-presenting cells, mast cells and microglia. HPGDS converts PGH2 into PGD2, a mediator thought to play a pivotal role in airway allergy and inflammatory processes (PMID: 16624958). HPGDS deficiency is a critical factor that impedes cutaneous wound healing in diabetes. Overexpressing Hpgds in ADSCs could be an effective way to accelerate DW healing by alleviating inflammatory cells recruitment and increasing M2 polarization in the proliferation phase (PMID: 35922870). HPGDS is a cytosolic homodimer of 26 kDa subunits which reveal a well-defined active site, enabling a structure-based design of inhibitors (PMID: 24900177).

Protocols

Product Specific Protocols
WB protocol for HPGDS antibody 22522-1-APDownload protocol
IHC protocol for HPGDS antibody 22522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Immunity

Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.

Authors - Zhen Xiong
humanWB

EMBO Mol Med

Tranilast directly targets NLRP3 to treat inflammasome-driven diseases.

Authors - Yi Huang
mouseWB

Molecules

Integrative Analysis of Proteomics and Metabolism Reveals the Potential Roles of Arachidonic Acid Metabolism in Hypoxia Response in Mouse Spleen

Authors - Yujing Guo