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PGM2 Polyclonal antibody

PGM2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11022-1-AP

Synonyms

Glucose phosphomutase 2, MSTP006, PGM 2, PGM2, Phosphodeoxyribomutase, phosphoglucomutase 2, Phosphopentomutase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGM2 Polyclonal antibody (11022-1-AP)

    WB analysis using 11022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11022-1-AP (PGM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PGM2 Polyclonal antibody (11022-1-AP)

    WB analysis of human brain using 11022-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11022-1-AP (PGM2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PGM2 Polyclonal antibody (11022-1-AP)

    WB analysis of HEK-293 using 11022-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11022-1-AP (PGM2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PGM2 Polyclonal antibody (11022-1-AP)

    IP experiment of HEK-293 using 11022-1-AP

    IP result of anti-PGM2 (IP:11022-1-AP, 4ug; Detection:11022-1-AP 1:200) with HEK-293 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PGM2 Polyclonal antibody (11022-1-AP)

    IHC staining of human pancreas cancer using 11022-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11022-1-AP (PGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PGM2 Polyclonal antibody (11022-1-AP)

    IHC staining of human pancreas cancer using 11022-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11022-1-AP (PGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PGM2 Polyclonal antibody (11022-1-AP)

    IHC staining of human pancreas cancer using 11022-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11022-1-AP (PGM2 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, fetal human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11022-1-AP targets PGM2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGM2 fusion protein Ag1488
Full Name phosphoglucomutase 2
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC010087
Gene Symbol PGM2
Gene ID (NCBI) 55276
RRIDAB_2161422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PGM2 antibody 11022-1-APDownload protocol
IHC protocol for PGM2 antibody 11022-1-APDownload protocol
IP protocol for PGM2 antibody 11022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Metab

Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions

Authors - Owen S Skinner
  • KO Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Patricia (Verified Customer) (02-25-2020)

Works well. No non-specific bands.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer