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PGM2L1 Polyclonal antibody

PGM2L1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13942-1-AP

Synonyms

BM32A, PGM2L1, phosphoglucomutase 2 like 1, PMMLP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGM2L1 Polyclonal antibody (13942-1-AP)

    WB analysis using 13942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13942-1-AP (PGM2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PGM2L1 Polyclonal antibody (13942-1-AP)

    WB analysis of mouse skeletal muscle using 13942-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13942-1-AP (PGM2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PGM2L1 Polyclonal antibody (13942-1-AP)

    WB analysis using 13942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13942-1-AP (PGM2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PGM2L1 Polyclonal antibody (13942-1-AP)

    WB analysis of mouse skeletal muscle using 13942-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13942-1-AP (PGM2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PGM2L1 Polyclonal antibody (13942-1-AP)

    IP experiment of mouse brain using 13942-1-AP

    IP result of anti-PGM2L1 (IP:13942-1-AP, 4ug; Detection:13942-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PGM2L1 Polyclonal antibody (13942-1-AP)

    IHC staining of human gliomas using 13942-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13942-1-AP (PGM2L1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PGM2L1 Polyclonal antibody (13942-1-AP)

    IHC staining of human gliomas using 13942-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13942-1-AP (PGM2L1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13942-1-AP targets PGM2L1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGM2L1 fusion protein Ag5019
Full Name phosphoglucomutase 2-like 1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC059360
Gene Symbol PGM2L1
Gene ID (NCBI) 283209
RRIDAB_2299394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PGM2L1 antibody 13942-1-APDownload protocol
IHC protocol for PGM2L1 antibody 13942-1-APDownload protocol
IP protocol for PGM2L1 antibody 13942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols