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  • KD/KO Validated

PI3 Kinase p110 Alpha Monoclonal antibody

PI3 Kinase p110 Alpha Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig, Rabbit, rabbit and More (6)

Applications

WB, RIP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1F6A7

Cat no : 67071-1-Ig

Synonyms

p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110 (alpha), PI3K p110(alpha), PIK3CA, PtdIns 3 kinase subunit alpha



Tested Applications

Positive WB detected inU2OS cells, pig brain tissue, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67071-1-Ig targets PI3 Kinase p110 Alpha in WB, RIP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit, rabbit
Cited Reactivityhuman, mouse, rat, monkey, fish, bovine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p110 Alpha fusion protein Ag17392
Full Name phosphoinositide-3-kinase, catalytic, alpha polypeptide
Calculated Molecular Weight 1068 aa, 124 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC113601
Gene Symbol PIK3CA
Gene ID (NCBI) 5290
RRIDAB_2882380
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p110 Alpha antibody 67071-1-IgDownload protocol
IHC protocol for PI3 Kinase p110 Alpha antibody 67071-1-IgDownload protocol
IF protocol for PI3 Kinase p110 Alpha antibody 67071-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Hazard Mater

Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis.

Authors - Jing Qin
mouseIHC

Cancer Lett

PI3K inhibitor impairs tumor progression and enhances sensitivity to anlotinib in anlotinib-resistant osteosarcoma.

Authors - Chenglong Chen
humanWB

Oxid Med Cell Longev

Transcriptional Patterns of Lower-Grade Glioma Patients with Distinct Ferroptosis Levels, Immunotherapy Response, and Temozolomide Sensitivity.

Authors - Zewei Tu
mouseWB

Oxid Med Cell Longev

Microbiota from Exercise Mice Counteracts High-Fat High-Cholesterol Diet-Induced Cognitive Impairment in C57BL/6 Mice

Authors - Rui Li
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
ratWB

Sci Total Environ

PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism.

Authors - Mei Ha

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iru (Verified Customer) (02-18-2021)

Not sure what the band is, I saw a clear band in between 38 and 50KD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Fallopian tube epithelial cells
PI3 Kinase p110 Alpha Antibody Western Blot validation (1:2000 dilution) in Fallopian tube epithelial cells (Cat no:67071-1-Ig)