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  • KD/KO Validated

PIK3IP1 Polyclonal antibody

PIK3IP1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16826-1-AP

Synonyms

HGFL, hHGFL(S), PIK3IP1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse liver tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16826-1-AP targets PIK3IP1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIK3IP1 fusion protein Ag10427
Full Name phosphoinositide-3-kinase interacting protein 1
Calculated Molecular Weight 263 aa, 28 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC011049
Gene Symbol PIK3IP1
Gene ID (NCBI) 113791
RRIDAB_2163333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3IP1(Phosphoinositide-3-kinase-interacting protein 1) is also named as HGFL. The class IA phosphoinositol-3-kinases (PI3Ks) regulate important cellular processes such as proliferation, growth, survival, motility and metabolism. PIK3IP1 is a transmembrane protein that possesses a region in its intracellular domain that shares homology with the p85 regulatory subunit of PI3K. It has 5 isoforms produced by alternative splicing with the MW of 11, 18, 19, 25,28 kDa. PIK3IP1 undergoes N- and O-linked amino acid glycosylation. The major glycosylated form of PIK3IP1 migrates at about 43 kDa in western blot analysis, while the unglycosylated form migrates at 37 kDa. It is also detected a 65 kDa variant in HepG2 cell lysate that PIK3IP1 is indeed membrane bound and that these variants arise from alternative splicing and/or post-translational processing events such as enzymatic proteolysis and/or glycosylation. (PMID:18632611).

Protocols

Product Specific Protocols
WB protocol for PIK3IP1 antibody 16826-1-APDownload protocol
IP protocol for PIK3IP1 antibody 16826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Genet

Inactivating CUX1 mutations promote tumorigenesis.

Authors - Chi C Wong

Cell

Distinct Regulation of Th17 and Th1 Cell Differentiation by Glutaminase-Dependent Metabolism.

Authors - Marc O Johnson
mouseWB

Nat Commun

Dual blockade of the lipid kinase PIP4Ks and mitotic pathways leads to cancer-selective lethality.

Authors - Mayumi Kitagawa
  • KD Validated
mouseWB

J Immunol

Cutting Edge: Foxp1 Controls Naive CD8+ T Cell Quiescence by Simultaneously Repressing Key Pathways in Cellular Metabolism and Cell Cycle Progression.

Authors - Hairong Wei
mouseWB

PLoS One

Pik3ip1 Modulates Cardiac Hypertrophy by Inhibiting PI3K Pathway.

Authors - Hong Ki Song
  • KD Validated
chickenWB,CoIP

Exp Cell Res

Suppression of PI3K signaling is linked to autophagy activation and the spatiotemporal induction of the lens organelle free zone.

Authors - Rifah Gheyas