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  • KD/KO Validated

PINX1 Polyclonal antibody

PINX1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12368-1-AP

Synonyms

LPTL, LPTS, PIN2 interacting protein 1, Pin2 interacting protein X1, PINX1, Protein 67 11 3, TRF1 interacting protein 1



Tested Applications

Positive WB detected inJurkat cells, A431 cells, C6 cells, NIH/3T3 cells, HeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12368-1-AP targets PINX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PINX1 fusion protein Ag3043
Full Name PIN2-interacting protein 1
Calculated Molecular Weight 328 aa, 37 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC015479
Gene Symbol PINX1
Gene ID (NCBI) 54984
RRIDAB_2164405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PINX1, also named as LPTL, LPTS and Protein 67-11-3, is a Liver-related putative tumor suppressor. It belongs to the PINX1 family. PINX1 is a microtubule-binding protein essential for faithful chromosome segregation. It mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. PINX1 inhibits telomerase activity. It may inhibit cell proliferation and act as tumor suppressor. PINX1 has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, but the expression status in epithelial ovarian tumors has not been investigated.

Protocols

Product Specific Protocols
WB protocol for PINX1 antibody 12368-1-APDownload protocol
IF protocol for PINX1 antibody 12368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

Anthracyclines disrupt telomere maintenance by telomerase through inducing PinX1 ubiquitination and degradation.

Authors - Zhang B B
  • KD Validated
humanIHC

Mol Cancer

The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway.

Authors - Xiao-Peng Tian
humanWB,IHC

Am J Cancer Res

PINX1 promotes malignant transformation of thyroid cancer through the activation of the AKT/MAPK/β-catenin signaling pathway.

Authors - JiHoon Kang
  • KD Validated
humanWB,IHC

Mol Cancer

PinX1 suppresses bladder urothelial carcinoma cell proliferation via the inhibition of telomerase activity and p16/cyclin D1 pathway.

Authors - Liu Jian-Ye JY
humanWB,IHC

Cancer Lett

PMID: 25045845

Authors - Xiao-Peng Tian
humanWB,IHC

Cancer Sci

Decreased expression of PinX1 protein is correlated with tumor development and is a new independent poor prognostic factor in ovarian carcinoma.

Authors - Cai Mu-Yan MY