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  • Featured Product
  • KD/KO Validated

PIP5K3 Polyclonal antibody

PIP5K3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Dot Blot, ELISA

Conjugate

Unconjugated

Cat no : 13361-1-AP

Synonyms

CFD, FAB1, KIAA0981, PIKFYVE, PIP5K, PIP5K3, PIPkin III, Type III PIP kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using PIP5K3 Polyclonal antibody (13361-1-AP)

    WB analysis of mouse lung using 13361-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PIP5K3 Polyclonal antibody (13361-1-AP)

    WB analysis using 13361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PIP5K3 Polyclonal antibody (13361-1-AP)

    WB analysis using 13361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using PIP5K3 Polyclonal antibody (13361-1-AP)

    WB analysis of mouse lung using 13361-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PIP5K3 Polyclonal antibody (13361-1-AP)

    IHC staining of human liver cancer using 13361-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PIP5K3 Polyclonal antibody (13361-1-AP)

    IHC staining of human liver cancer using 13361-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13361-1-AP targets PIP5K3 in WB, IHC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIP5K3 fusion protein Ag4177
Full Name phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
Calculated Molecular Weight 451 aa, 50 kDa, 237 kDa
Observed Molecular Weight 237 kDa
GenBank Accession NumberBC032389
Gene Symbol PIP5K3
Gene ID (NCBI) 200576
RRIDAB_10638310
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIP5K3 antibody 13361-1-APDownload protocol
IHC protocol for PIP5K3 antibody 13361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation.

Authors - Katharina Fitzian
  • KD Validated
View Article
humanWB

Elife

Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.

Authors - Sai Srinivas Panapakkam Giridharan
View Article
humanIHC

Front Oncol

TRIM68, PIKFYVE, and DYNLL2: The Possible Novel Autophagy- and Immunity-Associated Gene Biomarkers for Osteosarcoma Prognosis.

Authors - Jie Jiang
View Article

Eur J Pharmacol

Inhibition of c-Rel DNA binding is critical for the anti-inflammatory effects of novel PIKfyve inhibitor.

Authors - Masaomi Terajima
View Article
mouseDot Blot

BMC Immunol

miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment.

Authors - Jingsong Cao
View Article
humanWB

Autophagy

Mitochondrial respiratory chain deficiency inhibits lysosomal hydrolysis.

Authors - Lorena Fernandez-Mosquera
View Article