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PIP5KL1 Polyclonal antibody

PIP5KL1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 17547-1-AP

Synonyms

PI(4)P 5 kinase like protein 1, PIP5KL1, PIPKH

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PIP5KL1 Polyclonal antibody (17547-1-AP)

    WB analysis of HEK-293 using 17547-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17547-1-AP (PIP5KL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PIP5KL1 Polyclonal antibody (17547-1-AP)

    WB analysis of human brain using 17547-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17547-1-AP (PIP5KL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PIP5KL1 Polyclonal antibody (17547-1-AP)

    IF Staining of HEK-293 using 17547-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 17547-1-AP (PIP5KL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17547-1-AP targets PIP5KL1 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIP5KL1 fusion protein Ag11653
Full Name phosphatidylinositol-4-phosphate 5-kinase-like 1
Calculated Molecular Weight 22 kDa, 44 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC042184
Gene Symbol PIP5KL1
Gene ID (NCBI) 138429
RRIDAB_2252440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIP5KL1 antibody 17547-1-APDownload protocol
IF protocol for PIP5KL1 antibody 17547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols