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PKC Alpha Monoclonal antibody

PKC Alpha Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D11F4

Cat no : 66421-1-Ig

Synonyms

AAG6, PKC A, PKC alpha, PKCA, PKCα, PRKACA, PRKCA, Protein kinase C alpha type, protein kinase C, alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PKC Alpha Monoclonal antibody (66421-1-Ig)

    WB analysis of HeLa using 66421-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC alpha antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PKC Alpha Monoclonal antibody (66421-1-Ig)

    WB analysis of HEK-293 using 66421-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC alpha antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using PKC Alpha Monoclonal antibody (66421-1-Ig)

    WB analysis of C6 using 66421-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC alpha antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using PKC Alpha Monoclonal antibody (66421-1-Ig)

    WB analysis of RAW 264.7 using 66421-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC alpha antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PKC Alpha Monoclonal antibody (66421-1-Ig)

    IHC staining of human lung cancer using 66421-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66421-1-Ig (PKC alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PKC Alpha Monoclonal antibody (66421-1-Ig)

    IHC staining of human lung cancer using 66421-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66421-1-Ig (PKC alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKC Alpha Monoclonal antibody (66421-1-Ig)

    IF Staining of HeLa using 66421-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKC Alpha antibody (66421-1-Ig, Clone: 1D11F4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKC Alpha Monoclonal antibody (66421-1-Ig)

    IF Staining of HeLa using 66421-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Alpha antibody (66421-1-Ig, Clone: 1D11F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKC Alpha Monoclonal antibody (66421-1-Ig)

    IF Staining of HeLa using 66421-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66421-1-Ig(PKC alpha antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using PKC Alpha Monoclonal antibody (66421-1-Ig)

    FC experiment of HeLa using 66421-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Alpha (66421-1-Ig, Clone:1D11F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, C6 cells, RAW 264.7 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66421-1-Ig targets PKC Alpha in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PKC Alpha fusion protein Ag17275
Full Name protein kinase C, alpha
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC109274
Gene Symbol PRKCA
Gene ID (NCBI) 5578
RRIDAB_2881793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Protocols

Product Specific Protocols
WB protocol for PKC Alpha antibody 66421-1-IgDownload protocol
IHC protocol for PKC Alpha antibody 66421-1-IgDownload protocol
IF protocol for PKC Alpha antibody 66421-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

Arsenic interferes with spermatogenesis involving Rictor/mTORC2-mediated blood-testis barrier disruption in mice

Authors - Xiujuan Li
View Article