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PLCG2 Monoclonal antibody

PLCG2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8H5

Cat no : 67011-1-Ig

Synonyms

Phospholipase C gamma 2, Phospholipase C IV, PLC gamma 2, PLC IV, PLCG2, PLCγ2

Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using PLCG2 Monoclonal antibody (67011-1-Ig)

    WB analysis of Ramos using 67011-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using PLCG2 Monoclonal antibody (67011-1-Ig)

    WB analysis of Daudi using 67011-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PLCG2 Monoclonal antibody (67011-1-Ig)

    WB analysis of Raji using 67011-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PLCG2 Monoclonal antibody (67011-1-Ig)

    IHC staining of human tonsillitis using 67011-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67011-1-Ig (PLCG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PLCG2 Monoclonal antibody (67011-1-Ig)

    IHC staining of human tonsillitis using 67011-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67011-1-Ig (PLCG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inRamos cells, Raji cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67011-1-Ig targets PLCG2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLCG2 fusion protein Ag24355
Full Name phospholipase C, gamma 2 (phosphatidylinositol-specific)
Calculated Molecular Weight 148 kDa
Observed Molecular Weight 148 kDa
GenBank Accession NumberBC007565
Gene Symbol PLCG2
Gene ID (NCBI) 5336
RRIDAB_2882328
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholipase C-gamma2 (PLCG2) is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor.IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

Protocols

Product Specific Protocols
WB protocol for PLCG2 antibody 67011-1-IgDownload protocol
IHC protocol for PLCG2 antibody 67011-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Golgi retention and oncogenic KIT signaling via PLCγ2-PKD2-PI4KIIIβ activation in gastrointestinal stromal tumor cells

Authors - Yuuki Obata
  • KD Validated
View Article