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PLEKHA1 Polyclonal antibody

PLEKHA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10238-1-AP

Synonyms

PLEKHA1, TAPP 1, TAPP1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using PLEKHA1 Polyclonal antibody (10238-1-AP)

    WB analysis of human placenta using 10238-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10238-1-AP (PLEKHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PLEKHA1 Polyclonal antibody (10238-1-PBS)

    WB analysis of Jurkat using 10238-1-PBS

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10238-1-AP (PLEKHA1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human colon tissue using PLEKHA1 Polyclonal antibody (10238-1-AP)

    IHC staining of human colon using 10238-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10238-1-AP (PLEKHA1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PLEKHA1 Polyclonal antibody (10238-1-AP)

    IHC staining of human colon using 10238-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10238-1-AP (PLEKHA1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLEKHA1 Polyclonal antibody (10238-1-AP)

    IHC staining of human pancreas cancer using 10238-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10238-1-AP (PLEKHA1 antibody) at dilution of 1:100 (under 25x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLEKHA1 Polyclonal antibody (10238-1-AP)

    IF Staining of HeLa using 10238-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10238-1-AP (PLEKHA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman placenta tissue, Jurkat cells
Positive IHC detected inhuman colon tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10238-1-AP targets PLEKHA1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEKHA1 fusion protein Ag0318
Full Name pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC001136
Gene Symbol PLEKHA1
Gene ID (NCBI) 59338
RRIDAB_2163993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pleckstrin homology (PH) domain is commonly found in eukaryotic signaling proteins and possesses multiple functions including the abilities to bind inositol phosphates and various proteins. The tandem PH domain containing protein-1 (TAPP1) or PH domain containing-family A (phosphoinositide binding specific) member 1 (PLEKHA1), interacts strongly and specifically with phosphatidylinositol 3,4-trisphosphate [PtdIns(3,4)P(2)], which is one of the immediate breakdown products of PtdIns(3,4,5) P (3) and functions as a signalling molecule in insulin- and growth-factor-stimulated pathways. TAPP1 is also associated with the protein- tyrosine-phosphatase-like protein-1 (PTPL1 also known as FAP-1) and maintains PTPL1 in cytoplasm. By binding to PtdIns(3,4) P (2) and PTPL1, TAPP1 may regulate the membrane localization of PTPL1."

Protocols

Product Specific Protocols
WB protocol for PLEKHA1 antibody 10238-1-APDownload protocol
IHC protocol for PLEKHA1 antibody 10238-1-APDownload protocol
IF protocol for PLEKHA1 antibody 10238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Exp Med

Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.

Authors - Michelle E LeBlanc
View Article
mouse

Exp Eye Res

Function-first ligandomics for ocular vascular research and drug target discovery.

Authors - Xin Rong
View Article