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  • Featured Product
  • KD/KO Validated

PLOD2-Specific Polyclonal antibody

PLOD2-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

Cat no : 21214-1-AP

Synonyms

LH2, Lysyl hydroxylase 2, PLOD2-Specific, TLH

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    WB analysis of A431 using 21214-1-AP

    WB result of PLOD2-Specific antibody (21214-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2-Specific transfected A431 cells.

  • Western Blot (WB) analysis of various lysates using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    WB analysis using 21214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    WB analysis using 21214-1-AP

    DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    WB analysis of mouse liver using 21214-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    WB analysis of HT-1080 using 21214-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    WB analysis of mouse pancreas using 21214-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    WB analysis of mouse testis using 21214-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    IP experiment of mouse testis using 21214-1-AP

    IP result of anti-PLOD2-Specific (IP:21214-1-AP, 4ug; Detection:21214-1-AP 1:500) with mouse testis tissue lysate 5000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    IHC staining of human liver cancer using 21214-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    IHC staining of human liver cancer using 21214-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    IHC staining of human skeletal muscle using 21214-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    IHC staining of human skeletal muscle using 21214-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    IHC staining of human pancreas using 21214-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    IHC staining of human pancreas using 21214-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    IF Staining of HeLa using 21214-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD2-Specific antibody (21214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using PLOD2-Specific Polyclonal antibody (21214-1-AP)

    FC experiment of HeLa using 21214-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLOD2-Specific (21214-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inCaco-2 cells, A431 cells, DU 145 cells, HT-1080 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue, human pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21214-1-AP is PLOD2-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rabbit, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLOD2-Specific fusion protein Ag15614
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Calculated Molecular Weight 758 aa, 85 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC037169
Gene Symbol PLOD2
Gene ID (NCBI) 5352
RRIDAB_10733347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

Protocols

Product Specific Protocols
WB protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
IHC protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
IF protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
IP protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

J Clin Invest

Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.

Authors - Yulong Chen
View Article
human, mouseWB

Cancer Discov

Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis.

Authors - Eisinger-Mathason T S Karin TS
View Article
mouseWB

Arthritis Rheumatol

Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis.

Authors - Akio Yamamoto
View Article
humanWB

Proc Natl Acad Sci U S A

Intratumoral oxygen gradients mediate sarcoma cell invasion.

Authors - Daniel M Lewis
View Article
mouseIHC

Arthritis Rheum

Gene expression analysis of osteoarthritis synovium reveals elevation of transforming growth factor-beta responsive genes in osteoarthritis-related fibrosis.

Authors - Remst D F G DF
View Article
humanIF,WB,IHC

Cancer Lett

Hypoxic stellate cells of pancreatic cancer stroma regulate extracellular matrix fiber organization and cancer cell motility.

Authors - Masafumi Sada
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

RICHA (Verified Customer) (12-02-2019)

Excellent

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse fibroblast