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CoraLite®594-conjugated PLOD3 Monoclonal antibody

PLOD3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G12B8

Cat no : CL594-60058

Synonyms

LH3, Lysyl hydroxylase 3, PLOD3



Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60058 targets PLOD3 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PLOD3 fusion protein Ag1480
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Calculated Molecular Weight 738 aa, 85 kDa
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC011674
Gene Symbol PLOD3
Gene ID (NCBI) 8985
RRIDAB_2883412
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Protocols

Product Specific Protocols
IF protocol for CL594 PLOD3 antibody CL594-60058Download protocol
Standard Protocols
Click here to view our Standard Protocols