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PMM1 Polyclonal antibody

PMM1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 11252-2-AP

Synonyms

phosphomannomutase 1, PMM 1, PMM1, PMMH 22, PMMH22, Sec53

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PMM1 Polyclonal antibody (11252-2-AP)

    WB analysis of mouse brain using 11252-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PMM1 Polyclonal antibody (11252-2-AP)

    WB analysis of mouse brain using 11252-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PMM1 Polyclonal antibody (11252-2-AP)

    WB analysis of NIH/3T3 using 11252-2-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PMM1 Polyclonal antibody (11252-2-AP)

    WB analysis of human brain using 11252-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PMM1 Polyclonal antibody (11252-2-AP)

    WB analysis of HepG2 using 11252-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PMM1 Polyclonal antibody (11252-2-AP)

    IHC staining of human liver cancer using 11252-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PMM1 Polyclonal antibody (11252-2-AP)

    IHC staining of human liver cancer using 11252-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PMM1 Polyclonal antibody (11252-2-AP)

    IHC staining of human lung cancer using 11252-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PMM1 Polyclonal antibody (11252-2-AP)

    IHC staining of human lung cancer using 11252-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11252-2-AP targets PMM1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMM1 fusion protein Ag1782
Full Name phosphomannomutase 1
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC016818
Gene Symbol PMM1
Gene ID (NCBI) 5372
RRIDAB_2166971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PMM1, also named as PMMH22, belongs to the eukaryotic PMM family. It catalyzes the second step in the conversion of fructose-6P to GDP-mannoseis and is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, PMM1 may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain(PMID:18927083).

Protocols

Product Specific Protocols
WB protocol for PMM1 antibody 11252-2-APDownload protocol
IHC protocol for PMM1 antibody 11252-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Proteome Res

Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese.

Authors - Wegrzynowicz Michal M
View Article