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PMPCA Polyclonal antibody

PMPCA Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,IP,IF,ELISA

Conjugate

Unconjugated

Cat no : 26536-1-AP

Synonyms

Alpha MPP, INPP5E, KIAA0123, MPPA, P 55, PMPCA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PMPCA Polyclonal antibody (26536-1-AP)

    WB analysis using 26536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26536-1-AP (PMPCA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using PMPCA Polyclonal antibody (26536-1-AP)

    WB analysis of T-47D using 26536-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 26536-1-AP (PMPCA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MDA-MB-453s cells using PMPCA Polyclonal antibody (26536-1-AP)

    IP experiment of MDA-MB-453s using 26536-1-AP

    IP result of anti-PMPCA (IP:26536-1-AP, 4ug; Detection:26536-1-AP 1:500) with MDA-MB-453s cells lysate 2800ug.

  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using PMPCA Polyclonal antibody (26536-1-AP)

    IF Staining of T-47D using 26536-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 26536-1-AP (PMPCA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMDA-MB-453s cells, T-47D cells
Positive IP detected inMDA-MB-453s cells
Positive IF detected inT-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26536-1-AP is PMPCA Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMPCA fusion protein Ag19206
Full Name peptidase (mitochondrial processing) alpha
Calculated Molecular Weight 525 aa, 58 kDa
Observed Molecular Weight 50-58 kDa
GenBank Accession NumberBC132724
Gene Symbol PMPCA
Gene ID (NCBI) 23203
RRIDAB_2880545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PMPCA antibody 26536-1-APDownload protocol
IF protocol for PMPCA antibody 26536-1-APDownload protocol
IP protocol for PMPCA antibody 26536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich Ataxia cellular models.

Authors - Yi Na Dong
View Article