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  • KD/KO Validated

PNPT1 Polyclonal antibody

PNPT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14487-1-AP

Synonyms

3 5 RNA exonuclease OLD35, old 35, OLD35, PNPASE, PNPase 1, PNPase old 35, PNPT1, Polynucleotide phosphorylase 1



Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, HeLa cells, Jurkat cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human skin tissue, human spleen tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14487-1-AP targets PNPT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNPT1 fusion protein Ag5888
Full Name polyribonucleotide nucleotidyltransferase 1
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC053660
Gene Symbol PNPT1
Gene ID (NCBI) 87178
RRIDAB_2165820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.

Protocols

Product Specific Protocols
WB protocol for PNPT1 antibody 14487-1-APDownload protocol
IHC protocol for PNPT1 antibody 14487-1-APDownload protocol
IF protocol for PNPT1 antibody 14487-1-APDownload protocol
IP protocol for PNPT1 antibody 14487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Immunity

Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis

Authors - Rui Miao
  • KD Validated
mouseWB,IHC

Am J Hum Genet

A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.

Authors - von Ameln Simon S
  • KO Validated
mouseIF

Proc Natl Acad Sci U S A

EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells.

Authors - Tina Pangršič
mouseWB

EMBO J

CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.

Authors - Karolina Szczepanowska
humanWB

PLoS Genet

RNA binding protein FXR1-miR301a-3p axis contributes to p21WAF1 degradation in oral cancer.

Authors - Mrinmoyee Majumder
mouseIF

Front Cell Dev Biol

Differences in Calcium Clearance at Inner Hair Cell Active Zones May Underlie the Difference in Susceptibility to Noise-Induced Cochlea Synaptopathy of C57BL/6J and CBA/CaJ Mice.

Authors - Hongchao Liu