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POLR1D Polyclonal antibody

POLR1D Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12254-1-AP

Synonyms

AC19, hRPA19, POLR1C, POLR1D, RPA16, RPA9, RPAC2, RPC16, RPO1 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using POLR1D Polyclonal antibody (12254-1-AP)

    WB analysis of HeLa using 12254-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12254-1-AP (POLR1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using POLR1D Polyclonal antibody (12254-1-AP)

    WB analysis of Jurkat using 12254-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12254-1-AP (POLR1D antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using POLR1D Polyclonal antibody (12254-1-AP)

    WB analysis of mouse lung using 12254-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12254-1-AP (POLR1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using POLR1D Polyclonal antibody (12254-1-AP)

    IP experiment of HeLa using 12254-1-AP

    IP result of anti-POLR1D (IP:12254-1-AP, 4ug; Detection:12254-1-AP 1:500) with HeLa cells lysate 2600 ug.

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse lung tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12254-1-AP targets POLR1D in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR1D fusion protein Ag2904
Full Name polymerase (RNA) I polypeptide D, 16kDa
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC015319
Gene Symbol POLR1D
Gene ID (NCBI) 51082
RRIDAB_10640535
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLR1D antibody 12254-1-APDownload protocol
IP protocol for POLR1D antibody 12254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells

Authors - Chandreyee Datta
View Article