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POLR1D Polyclonal antibody

POLR1D Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 16678-1-AP

Synonyms

AC19, hRPA19, POLR1C, POLR1D, RPA16, RPA9, RPAC2, RPC16, RPO1 3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using POLR1D Polyclonal antibody (16678-1-AP)

    WB analysis of Jurkat using 16678-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16678-1-AP (POLR1D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLR1D Polyclonal antibody (16678-1-AP)

    WB analysis of HeLa using 16678-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16678-1-AP (POLR1D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using POLR1D Polyclonal antibody (16678-1-AP)

    WB analysis of HepG2 using 16678-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16678-1-AP (POLR1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR1D Polyclonal antibody (16678-1-AP)

    IF Staining of HepG2 using 16678-1-AP

    Immunofluorescent analysis of HepG2 cells using 16678-1-AP (POLR1D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16678-1-AP is POLR1D Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR1D fusion protein Ag10000
Full Name polymerase (RNA) I polypeptide D, 16kDa
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000889
Gene Symbol POLR1D
Gene ID (NCBI) 51082
RRIDAB_2878298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLR1D antibody 16678-1-APDownload protocol
IF protocol for POLR1D antibody 16678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols