PON2 Polyclonal antibody

PON2 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14379-1-AP

Synonyms

A esterase 2, Aromatic esterase 2, paraoxonase 2, PON 2, PON2, Serum aryldialkylphosphatase 2



Tested Applications

Positive WB detected inhuman liver tissue, L02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14379-1-AP targets PON2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PON2 fusion protein Ag5759
Full Name paraoxonase 2
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC046160
Gene Symbol PON2
Gene ID (NCBI) 5445
RRIDAB_2166330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PON2(Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).

Protocols

Product Specific Protocols
WB protocol for PON2 antibody 14379-1-APDownload protocol
IHC protocol for PON2 antibody 14379-1-APDownload protocol
IF protocol for PON2 antibody 14379-1-APDownload protocol
IP protocol for PON2 antibody 14379-1-APDownload protocol
FC protocol for PON2 antibody 14379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

FASEB J

Breaking the chain at the membrane: paraoxonase 2 counteracts lipid peroxidation at the plasma membrane.

Authors - Henning Hagmann
mouseWB

Am J Pathol

Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis.

Authors - Joe Myung Kuk MK
human,mouseIHC

Cells

Paraoxonase 2 (PON2) Deficiency Reproduces Lipid Alterations of Diabetic and Inflammatory Glomerular Disease and Affects TRPC6 Signaling

Authors - Henning Hagmann
mouseWB,IF

Biomed Pharmacother

GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes

Authors - Xueqi Chen